Standard Operating Procedure
Abstract
This is a Standard Operating Procedure (SOP) to biochemically modify, glycerolize and deglycerolize human red cells in the 800 ml primary polyvinyichioride (PVC) plastic collection bag system. Red blood cell concentrates stored at 4 C in the 800 ml primary plastic collection bag can be biochemically modified to increase the red cell 2,3 DPG and ATP levels; this procedure prepares indated-rejuvenated red cells (red cells stored at 4 C for 3 to 6 days are biochemically modified to prepare red-cells with 250% of normal 2,3 DPG and with 175% of normal ATP levels and red cells stored for 7 to 35 days are treated to prepare red cells with 150% of normal 2,3 DPG and 175% of normal ATP levels). Outdated red cells stored at 4 C for 36 to 38 days can be biochemically modified to increase the 2,3 DPG level to 150% of normal and the ATP level to 175% of normal; this procedure prepares outdated-rejuvenated red cells. Biochemically modified indated-frozen-thawed-washed red cells have improved oxygen transport function and biochemically modified outdated frozen-thawed-washed red cells have normal oxygen transport function. After the rejuvenation procedure, the red cells are concentrated, the supernatant removed, and the red cells glycerolized prior to freezing at -80 C.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1997
- Accession Number
- ADA360322