Development and Use of Novel Tools to Directly Screen for Substrates of Cyclin Dependent Kinases
Abstract
Cell division is a central and primary biological function. Many problems in biology and medicine center around cell division, but without a knowledge of the mechanism by which division is controlled, these problems are difficult to attack. The major transitions of the cell cycle are is controlled by a protein called a cyclin dependent kinase, or Cdk. The cell cycles of both yeast and human cells are controlled by a Cdk. Using the yeast two hybrid system I have isolated many novel Cdk interacting proteins in yeast, and these may be instrumental in controlling cell cycle progression. I have also isolate two exciting proteins which can only interact with a substrate trapping mutant of a yeast Cdk. These may be Cdk new substrates. I have also used the genomic technology of "Gene Chips", to define the complete set of cell cycle regulated genes in yeast, and to characterize their controlling promoter elements. These two projects have yielded novel and exciting results, of interest to both the yeast and cell cycle fields.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADA360338
Entities
People
- Gavin J. Sherlock
Organizations
- Cold Spring Harbor Laboratory