Rejuvenation and Freezing of Additive-Preserved Red Blood Cells in the 1000 ML-600 ML Polyvinyl Chloride Freezing Bay System Stored for Up to 42 Days at 4 C Prior to Rejuvenation and Glycerolization Using 40% W/V Glycerol and Storage at -80 C, Washed in the Haemonetics Blood Processor 115, and Stored at 4 C for up to 24 Hours Prior to Transfusion
Abstract
This is a Standard Operating Procedure (Sop) to rejuvenate, glycerolize and deglycerolize human red cells collected into anticoagulant preservative (additive) systems. Current red blood cell additive systems include CPD-ADSOL (Fenwal Co.), CPD-Optisol (Terumo), and CP2D- Nutricel (MedSep) . Red blood cells collected into these systems can be stored at 4 deg C for up to 42 days prior to rejuvenation and freezing in the 1000-600 ml polyvinyl chloride (PVC) plastic bag. The rejuvenated, glycerolized red cells are concentrated and the supernatant glycerol removed prior to freezing. The rejuvenated, glycerolized red cells are washed in the Haemonetics Blood Processor 115.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 14, 1997
- Accession Number
- ADA360394
Entities
Organizations
- Boston University