Isolation of Genomic Targets for the Suspected DNA-Binding Protein BRCA1
Abstract
BRCA1, named after breast cancer gene 1, is a human breast-ovarian cancer susceptibility gene whose mutation, deletion or under-expression has been implicated in most breast cancer. To better understand the function of the crucial BRCA1 protein and specifically, to explore the hypothesis that BRCA1 is a DNA-binding protein, we have begun an exciting line of research to isolate genomic targets for BRCA1 protein binding. If our hypothesis is correct, this research may help discover the identity of genes whose expression is regulated by BRCA1 and thereby provide important new insights into BRCA1-mediated tumor suppression. To accomplish these goals, we proposed to use an immunopurification strategy to capture BRCA1-DNA complexes and test isolated DNA fragments for their ability to bind BRCA1 in vitro. During the first year of funding we have laid the groundwork for successive years by raising specific antibodies to BRCA1 that can be used for immunopurifcation of BRCA1 targets. Our antibodies are an important resource for our DoD-sponsored research and also for other avenues of BRCA1 research in my laboratory. In summary, we have developed specific antibodies to the BRCA1 protein which will be used to isolate, by immunopurification, likely targets for BRCA1 binding.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA360431
Entities
People
- Simon A. Smith
Organizations
- University of Kentucky