Structural Studies of Metalloprotein Design
Abstract
This project utilized the zinc enzyme human carbonic anhydrase II (CAII) as a paradigm for dissecting and understanding the structural basis of protein transition metal recognition and discrimination. We have successfully redesigned the zinc binding site of CAII so that protein-metal affinity can be modulated by design, by factors of 10 from micromolar to femtomolar affinity. Additionally, our work has illuminated the structural basis of metal binding kinetics, a prerequisite for the development and deployment of a metal ion biosensor.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 02, 1999
- Accession Number
- ADA360687
Entities
People
- David W. Christianson
Organizations
- University of Pennsylvania