Biosynthesis of 3-Dimethylsulfoniopropionate in Marine Algae
Abstract
The aim of this research was to investigate the steps in the biosynthesis pathway of 3-dimethylsulfoniopropionate (DMSP) in marine algal groups. DMSP is the biogenic precursor of atmospheric dimethylsulfide (DMS) gas. A combination of in-vivo radioactive and stable isotope labeling and enzyme assay was employed. The pathway was studied in the intertidal green macroalga Enteroinorpha intestinalis and in three diverse microalgae (Tetraselmis sp., Emiliania huxleyi and Melosira nummuloides. Evidence was obtained for the following pathway in all cases: methionine 4-methylthio-2-oxobutyrate 4- methylthio-2-hydroxybutyrate 4-dimethylsuifonio-2-hydroxybutyrate DMSP. The enzymes mediating the first three steps, an aminotransferase, a reductase and a methyltransferase, were detected in extracts of E. intestinalis. In-vivo 180-labeling data indicated that the final step is mediated by an oxygenase.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 05, 1999
- Accession Number
- ADA360698
Entities
People
- Andrew D. Hanson
Organizations
- University of Florida