Efficiency of PBN to Trap 3-CAR in B6C3F1 Mouse Liver Slices: An EPR Study.
Abstract
Several active Air Force bases and bases targeted for closure have measurable quantities of the halocarbon trichloroethylene (TCE) in the soil and ground water. TCE causes liver tumors in B6C3F1 mice. As part of the process to develop an environmental health effects criteria for base clean up a study of the effects of TCE induced free radicals in liver slices had been performed. Radicals of TCE can be identified by electron paramagnetic resonance (EPR) using the spin trap N-tert-butyl-a phenyl nitrone (PBN). To quantitate the radicals detected a stable spin label 2,2,5,5,-tetramethyl-l-pyrolidinyl oxy-3-carboxamide (3-CAR) is used as a standard. The trapping efficiency of PBN to detect 3-CAR was studied. Known amounts 3-CAR was added to non-TCE treated liver slices of B6C3Fl mice. These liver slices were lyophilized with and without 10 mM PBN. Using the assumption that the double integration of the 3-CAR spectrum in lyophilized is proportional to the number of radicals present and the double integration of the spectrum of PBN-3-CAR in lyophilized liver is proportional to the number of radicals trapped, the efficiency of PBN to trap 3-CAR was determined to be 33 %.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1995
- Accession Number
- ADA360715
Entities
People
- Brian Hutchens
- Linda Steel-goodwin
Organizations
- Armstrong Laboratory