Uses of EPR/Spin Labeling as a Biomarker
Abstract
Recently protein receptor changes has attracted interest for use as biomarkers in environmental toxicology. Insulin and transferrin which control cell growth were chosen in this study. There were two aims: first, to measure insulin and transferrin receptor binding using spin-labeled insulin and second; basic research on changes in receptor binding of human erythrocytes after exposure to radiation and chemicals likely to be encountered by military personnel in operational or combat scenarios. Insulin and transferrin were spin-labeled and incubated with red blood cells exposed to y-radiation or chemicals (ammonium dinitramide or mustard gas), the cells were centrifuged and receptor binding measured from the EPR spectra using a Bruker ESP3O()E and EMS 104 spectrometer. Transferrin and insulin receptor binding was decreased with ammonium dinitramide. Transferrin receptors are only present in immature erythrocytes. Insulin receptor binding was decreased by y-irradiation and increased by mustard gas. Irradiated mustard gas did not increase insulin receptor binding in red blood cells. As most cells contain insulin receptors, insulin receptor binding is likely to be useful for risk assessment efforts and the spin label technique could be developed as a clinical tool not just for insulin but for other compounds with specific protein receptors on cell membranes.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1996
- Accession Number
- ADA360791
Entities
People
- A. J. Carmichael
- C. Flemming
- F. C. Music
- K. W. Dean
- L. Steel-goodwin
Organizations
- Armstrong Laboratory