Effects of Ammonium Dinitramide in Human Liver Slices: An EPR/Spin Trapping Study.
Abstract
Ammonium dinitrarnide (NH4NNO22 ADN) is a high energy oxidizer currently under study by the US Air Force as a replacement for ammonium perchlorate (AP). ADN is believed to be more environmentally safe than AP. EPR/spin trapping studies with the spin trap N-tert-butyl-a-phenyl nitrone (PBN 10mM) for 5 min in the presence of ADN yielded an over lapping spin adduct EPR spectra. It consisted of a 1:2:2:1 quartet initially which was computer simulated using hyperfine coupling constants, an=ah= 1.42 mT. After 60 min another spectra was also formed consisting of a triplet of doublets with hyperfine coupling constants an= 1.65 mT and ah =0.4 mT. These radicals are similar to the effects of phosgene on PBN. Precision cut human liver slices were incubated with the spin trap PBN for 5 or 60 min with and without the 10 mM ammonium dinitramide (NH4NNO22 ADN). The liver slices was homogenized, frozen in liquid nitrogen and lyophilized. ADN oxidized the PBN in human liver within 5 min exposure to form a yellow hydroscopic compound which was no longer paramagnetic. The reaction of ADN with liver caused acute injury.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1995
- Accession Number
- ADA360858
Entities
People
- A. J. Carmichael
- D. M. Pace
- K. W. Dean
- L. Steel-goodwin
Organizations
- Armstrong Laboratory