The Effect of DNA Methylation on IGF2 Expression.

Abstract

Our hypothesis is that DNA methyl transferase expression is inversely correlated with IGF-II expression in normal and tumor tissues. That is, tumors with high DNA methyl transferase expression will imprint IGF-II, resulting in reduced IGF-II expression, while tumors with low DNA methyl transferase will have higher relative IGF-II expression. We have demonstrated that DNA methyl transferase expression can be measured readily using RNase protection assay in representative breast cancer cell lines. Sahana Kaup is continuing her graduate studies based on her findings along the outline discussed above. At the present time, her preliminary data have not confirmed her fundamental hypothesis, but further experiments which are currently underway will determine whether or not her original hypothesis remains worthy of investigation.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1998
Accession Number
ADA361324

Entities

People

  • Kevin J. Cullen

Organizations

  • Georgetown University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Chemical Compounds
  • Deoxyribonucleic Acids
  • Epithelial Cells
  • Laboratory Animals
  • Materials
  • Methylation
  • Neoplasms
  • Ovarian Cancer
  • Phosphodiesterases
  • Recombinant Dna
  • Transferases
  • Tumor Cell Line

Readers

  • Molecular and genetic basis of cancer.
  • Systems Analysis and Design