Complement Activation By Liposome-Encapsulated Hemoglobin in Vitro: The Role of Endotoxin Contaminative.

Abstract

Incubation of liposome-encapsulated Hb (LEH) with rat serum at 37C led to accelcelerated decay of serum complement (C) hemolytic activity (CH50). Empty liposomes (L) caused less expressed decrease of CH50, whereas free Hb had no effect on C hemolytic activity. The LEH- and L-induced increase in C consumption was unlikely due to endotoxin (LPS) contamination, as spiking of rat serum with LPS caused reduction in CH50 only at levels significantly higher than detectable in LEH or L. The LPS- induced C consumption was not potentiated by free hemoglobin.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1995
Accession Number
ADA361496

Entities

People

  • Alan S. Rudolph
  • Carl R. Alving
  • Janos Szebeni
  • Nabila M. Wassef

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biochemistry
  • Biomedical Research
  • Blood
  • Blood Cells
  • Blood Substitutes
  • Cells
  • Chemical Compounds
  • Chemistry
  • Contamination
  • Diseases And Disorders
  • Dwell Time
  • Dynamic Range
  • Hemoglobin
  • Incubation
  • Materials
  • Rodents
  • Synthetic Membranes

Fields of Study

  • Biology
  • Medicine

Readers

  • Mathematics or Statistics
  • Molecular and Cellular Biochemistry
  • Rocket Propulsion.