Identification and Localization of Genes which Restore Senescence in Breast Cancer Cells

Abstract

We have identified a gene on chromosome 16 which restores senescence to human and rat mammary tumor cells by functional complementation. Initial efforts at mapping, using PCR based analysis of rat revertant clones arising from senescent colonies, revealed that the senescence gene was located within a 3-7 cM consensus deletion region at 16q24.3. A yeast artificial chromosome (YAC) contig of this consensus deletion region was obtained and analyzed for marker content. Two overlapping YACs, 792 E-1 and 624 H-7, spanning this region were retrofitted with a selectable marker. Transfer of these two YACs by spheroplast fusion restored senescence to immortal rat ovarian tumor cells. Indirect transfer of retrofitted YAC 792-E-1 by microcell mediated chromosome transfer restored senescence to immortal human and rat mammary tumor cells.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1998
Accession Number
ADA361520

Entities

People

  • Deepthi Reddy

Organizations

  • Temple University

Tags

DTIC Thesaurus Topics

  • Biological Aging
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Cells (Biology)
  • Chemistry
  • Chromosomes
  • Fungi
  • Genes
  • Genetic Phenomena
  • Genetic Structures
  • Genetics
  • Identification
  • Neoplasms
  • Pcr Testing
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and genetic basis of cancer.