Ret Receptor: Functional Consequences of Oncogenic Rearrangements

Abstract

The ret/ptc2 gene was cloned from human papillary thyroid carcinomas, and is the product of a reciprocal chromosomal rearrangement, translocation event between the cAMP dependent protein kinase regulatory subunit I alpha (RI alpha) and the tyrosine kinase domain of the Ret receptor. Ret/ptc2 is a dimer which is autophosphorylated, soluble, and constitutively active. We generated a computer model of the Ret/ptc2 kinase domain, expressed and purified Ret/ptc2, developed a kinase assay to test a variety of peptide substrates and a microinjection assay to compare the structure function relationship of Ret/ptc2 mutants. A number of tyrosine residues within the Ret/ptc2 kinase domain as well as the RI alpha dimerization domain are critical for eliciting a mitogenic response.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1998
Accession Number
ADA361697

Entities

People

  • Susan S. Taylor

Organizations

  • University of California, San Diego

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Amino Acids
  • Cell Line
  • Cell Membrane Structures
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Fungi
  • Medical Personnel
  • Organic Chemistry
  • Proteins
  • Sugar Alcohols
  • Tyrosine

Fields of Study

  • Biology
  • Chemistry

Readers

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