Toxicity of Perfluoro Polyethers In Vitro.
Abstract
Two thermal and oxidatively stable perfluorinated polyether oils (FomblinR and GaldenR) were tested to determine if this class of compounds could be screened by in vitro methods to reduce cost, time and animal use in identifying suitable non-flammable oils for commercial and military use. Fomblin and Galden were found only to be soluble in other florinated solvents, such as freon and trifluoroacetic acid, that were inadequate as solvent vehicles in aqueous tissue culture medium. Due to its large molecular weight ( 4100 AMU) and insolubility in water, Fomblin could not be detected in aqueous medium by gas chromatography methods. Gas chromatographic analysis of Galden saturated medium indicated three peaks with retention times of 3.5, 6.1 and 9.0 min, respectively. Gas chromatography/Mass spectrometry analysis of these samples identified 4 peaks. The concentration of Galden in saturated culture medium over 72 h remained relatively constant at 84.9 + or - 3.0 mg/ml. However, the concentration of Galden in the culture medium of micro roller bottle cell exposure chambers containing or lacking primary rat hepatocytes was found to be 30 + or - 4 mg/ml after 24 h. Furthermore, the early retention time peak of Galden was not detected in these samples. No toxicity was observed in primary hepatocytes exposed to the highest concentration of Galden maintained in the culture medium of the micro rollerbottle chambers as determined by lactate dehydrogenase enzyme leakage. This study indicates that large molecular weight perfluorinated polyether compounds (Fomblins) that are insoluble in aqueous medium do not lend themselves to in vitro analysis. However, lower molecular weight material (Galdens) exhibiting some solubility in water can be assessed for toxicity in vitro methods.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1996
- Accession Number
- ADA362262
Entities
People
- Dan Pollard
- Marcia Ketcha
- Merry Walsh
- Nicholas Delraso