Regulation of the Multidrug Resistance-Associated Protein Gene by Estrogen.
Abstract
An observation was made that the mRNAs for two clones, #41 and #44, were rapidly repressed by estrogen. Clone #44 shared sequence homology with members of the multidrug resistance-associated protein (NRP) gene family. This raised the question of whether the MDR phenotype in breast cancer may in part be due to the loss of repression of MRP by estrogen when TAM is administered. The goals of this past year were to complete the identification of #41 and #44, to ascertain whether they are homologues of MRP, to verify their regulation by estrogen, and to begin testing whether the human MRP gene may also be regulated by estrogen. Significant progress has been made on most of these goals and a manuscript is in preparation on this work. In particular, we have shown that #44 is the chick orthologue of the human MRP1 gene, we have cloned an additional 1363 bp of #41 but data bases show no significant homologies with other cloned genes, the half lives of repression of the mRNAs for the two clones have been determined, and preliminary data suggest that the regulation of the chick MRP gene is probably post-transcriptiona.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA362659
Entities
People
- Michel M. Sanders
Organizations
- University of Minnesota