Fidelity of DNA Replication in Normal and Malignant Human Breast Cells
Abstract
In order to determine the degree to which the accumulation of mutations in breast cancer cells is due to a change in the fidelity of the cellular DNA replication machinery we have completed a series of experiments utilizing the multiprotein DNA synthesome isolated from malignant (cell culture and human tumors) and non-malignant (cell culture, benign human breast disease tissue, and disease-free human breast tissue) breast cells. The DNA synthesome has been extensively demonstrated to carry out full length DNA replication in vitro, and to accurately depict the DNA replication process as it occurs in the intact cell. By examining the fidelity of the DNA replication process carried out by the DNA synthesome from a number of breast cell types, we have demonstrated for the first time, that the cellular DNA replication machinery of malignant human breast cells is significantly more error-prone than that of non- malignant human breast cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA362661
Entities
People
- Jennifer W Sekowski
Organizations
- University of Maryland School of Medicine