Genomic Instability at Premalignant and Early Stages of Breast Cancer Development.
Abstract
Allelic imbalances and losses affecting the chromosome 16q arm appear to be early abnormalities in breast carcinogenesis since they were observed in a significant number of preinvasive lesions. The most common region of overlapping allelic losses spans the region 16q23.3 - q24.1, observed affected in 60 - 70% of in situ breast lesions. Positional cloning strategies are currently being employed to clone the putative tumor suppressor gene. We have defined approximately 300 kb of a homozygous deletion within the region q23.3 - q24. 1 of chromosome 16 in a breast cancer cell line. A total of 29 previously reported ESTs were mapped to this region. Five new cDNA clones were isolated and mapped. The analysis of 150 kb of the genomic sequence from this deletion showed 22 putative exons. The identification of this gene as well as the use of molecular markers from this chromosome region will provide tools of potential diagnostic-prognostic significance.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA362712
Entities
People
- C M Aldaz
Organizations
- The University of Texas MD Anderson Cancer Center