The Role of Phospholipase A2 and the Products of Its Action in the Regulation of Chondrocytes by Vitamin D3 Metabolites.
Abstract
Long hone formation, osseous repair, and potentially, guided tissue regeneration, all occur through endochondral bone formation, a process which involves chondrocyte differentiation, proliferation, hypertrophy, and mineralization Vitamin D3 metabolites have been shown to play a pivotal role in chondrocyte differentiation and are therefore essential for proper endochondral classification. In fact, the actions of the vitamin D3 metabolites are cell maturation-dependent with l,25-(OH)2D3 primarily affecting growth zone chondrocytes and 24,25-(OH)2D3 primarily affecting resting zone chondrocytes. Prior studies have demonstrated that 1,25-(OH)2D3 increases phospholipase A2 activity in growth zone chondrocytes, while 24,25-(OH)2D3 decreases phospholipase A2 activity in resting zone chondrocytes. In addition, these vitamin D3 metabolites mediate their effects in a cell maturation-dependent manner through activation of protein kinase C (PKC), an enzyme involved in cellular signal transduction. The aim of the present study was to examine the role of phospholipase A2 in mediating the activation of PKC by vitamin D3 metabolites.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADA364095
Entities
People
- Douglas B. Curry
Organizations
- University of Texas Health Science Center at San Antonio