Characterization of the Integrated Virus Detection System (IVDS) Using MS-2 Bacteriophage
Abstract
The detection and analysis of viruses have been goals of science for more than 70 years, following the first real evidence that a new type of microorganism was responsible for diseases in man and animals. These new microbes were smaller than bacteria, which have now been well documented, classified, and studied. Their small size made classifying the new microbes more difficult, and the field of virology has been advanced by biochemical techniques rather than by direct examination. Advancements in electron microscopy in more recent times have made advances in this area, and much has been reported on the physical features of more than 21 virus families. All these historic techniques are time consuming and require special knowledge and specialized chemicals and preparations to be successful. Capitalizing on the physical characteristics, it was possible to separate the families and count the individual viruses in a new and dramatic way, using easily obtained materials and simple-to-operate techniques. The only materials used in the Integrated Virus Detection System (IVDS) are a buffer, CO2 gas, and butanol. The work in this report presents the advances in the concentration and counting phases of IVDS. Results indicate a practical, easy to use device, which can count viruses in near real time. The JVDS can, in its final configuration, be expected to analyze a sample, count the viruses present, and give a preliminary identification for all viruses.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADA364117
Entities
People
- Charles H. Wick
- David M. Anderson
- Patrick E. Mccubbin
Organizations
- Edgewood Chemical Biological Center