Determination of the Crystal Structure of Human Zn-Alpha 2-Glycoprotein, A Protein Implicated in Breast Cancer

Abstract

Zn-alpha-2-glycoprotein (ZAG) is a soluble protein related to class I major histocompatibility complex (MHC) proteins. ZAG is present in most bodily fluids. In addition, ZAG accumulates in breast cysts as well as in 40% of breast carcinomas. ZAG stimulates lipid degradation in adipocytes and appears to be responsible of the massive loss of fat tissue happening in some patients with advanced cancer. We have solved the three-dimensional structure of human ZAG. ZAG is strikingly similar to class I MHC proteins but it does not bind Beta-2-microglobulin. A network of hydrogen bonds between ZAG's first domains and the last one stabilizes ZAG in the absence of Beta-2-microglobulin. ZAG carries a hydrophobic non-peptidic ligand in the homologous site to the peptide binding groove of other MHC molecules. We are studying the identity and role of this ligand in ZAG's function.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1998
Accession Number
ADA364341

Entities

People

  • Luis Sanchez

Organizations

  • California Institute of Technology

Tags

DTIC Thesaurus Topics

  • Blood
  • Breast Cancer
  • Cells
  • Chemical Compounds
  • Chemistry
  • Crystal Structure
  • Crystals
  • Cysts
  • Data Sets
  • Electron Density
  • Macromolecules
  • Materials
  • Molecules
  • Neoplasms
  • Proteins
  • Three Dimensional
  • X Rays

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