Response of Breast Cancer Cells to Hormonal Therapy: Quantitative in vivo NMR Studies
Abstract
Our research is directed at determining the specific metabolic pathways and the individual reactions affected by estrogen and TAM in both ER+ and ER- cells through the use of novel NMR methodology and quantitative analysis. In this report period, we have identified the primary metabolic pathways that are operative in ER+ cells. This was accomplished using a comprehensive metabolic model and NMR data from cell extracts. We have also performed preliminary studies necessary for the design and development of HFBR experiments. Investigations to characterize TAM and estrogen rescue response in ER+ cells were performed first in 96-well plates to determine simple dose response. Microcarrier culture, flask, and roller bottle studies were performed in order to demonstrate the ability and further develop methodology used to quantify breast cancer cell metabolism under varied treatment conditions. Metabolic fluxes were calculated for TAM-treated cells using NMR data from cell extracts and our metabolic model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA365454
Entities
People
- Douglas S Clark
Organizations
- University of California, Berkeley