Use of Genetic Suppressor Elements to Identify Potential Breast Tumor Suppressor Genes.
Abstract
ING1 is recently isolated breast cancer candidate tumor suppressor gene. In this study, structure and expression of the mouse ing1 gene were analyzed. Ing1 is transcribed from three differently regulated promoters. Each of the resulting transcripts represents two exons; they share a long common region encoded by a common exon and differ in their 5'-exon sequences encoded by alternative 5'-exons. Three alternative transcripts of ing1 encode two proteins one of which is a truncated version of the other. Protein sequences encoded by mouse and human ing1 are highly conserved. Expression of ing1 mRNA expression correlates with cell proliferation suggesting a connection between cell growth regulation and ing1 expression. Embryonic stem cells with heterozygous deletion of ing1 common exon are prepared as a first step in the generation of ing1 knockout mice. Analysis of ing1 knockout animals should give important information about the role ing1 might have in carcinogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA365474
Entities
People
- Marija Zeremski
Organizations
- University of Illinois at Chicago