Identification of Markers of the Invasive Phenotype in Human Breast Cancer
Abstract
Our goal is to identify genes involved in the development of the invasive phenotype as these may offer predictive markers and markers of risk of invasive disease in pre-invasive lesions. We have applied our tissue based strategy to directly identify differentially expressed genes between pre-invasive in-situ (DCIS) and adjacent early invasive tumor cell populations and have identified two promising candidate invasion' genes. To pursue the role of Psoriasin (S100 A7) we have generated a specific antibody for western blot and immunohistochemistry, transfected a breast cell line to study as a model MDA-MB-231 (Psoriasin), and determined that relatively higher psoriasin mRNA and protein correlates with the more invasive ER negative phenotype, within the range seen in invasive tumors. Lumican is a small leucine- rich proteoglycan, overexpressed by fibroblast-like cells, particularly immediately adjacent to in-situ elements, and at higher levels in association with several parameters indicative of more biologically aggressive breast tumors. It is also the most abundant proteoglycan within its stromal gene family. The pattern of upregulation and abnormal protein deposition within subregions of breast tumors, and its known role in cross linking of collagen in normal tissues, supports the hypothesis that changes in lumican expression may influence invasiveness.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA365482
Entities
People
- Peter H. Watson
Organizations
- University of Manitoba