Automated DNA Sizing by Atomic Force Microscopy.

Abstract

DNA sizing is one of the most widely used analytical methods in molecular biology and biochemistry, and it is a core method for genomic analysis. Many research, health care, and forensic applications rely on DNA sizing. Given the volume of DNA sizing done and the associated cost, an increasing need exists for extremely rapid, high- throughput, and inexpensive techniques. Essentially all DNA sizing today is performed by gel electrophoresis, although multiple efforts are under way to develop more effective technologies based on entirely new approaches. One such effort is described in this article: the use of atomic force microscopy in combination with pattern recognition software to perform DNA sizing. Initial results show that this approach can be compared with gel electrophoresis for some applications. Future work aims at developing a practical, high-throughput DNA sizing system.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1999
Accession Number
ADA365500

Entities

People

  • Fang Ye
  • Isaac N. Bankman
  • Jan H. Hoh
  • Roger H. Reeves
  • Thomas S. Spisz

Organizations

  • Air Force Research Laboratory

Tags

DTIC Thesaurus Topics

  • Accuracy
  • Chemical Analysis
  • Chemistry
  • Chromosomes
  • Gel Electrophoresis
  • Genetics
  • Health Care
  • Human Genome
  • Image Processing
  • Mass Spectrometry
  • Microscopes
  • Microscopy
  • Molecular Biology
  • Molecules
  • Pattern Recognition
  • Polymerase Chain Reaction
  • Proteins

Readers

  • Computational Modeling and Simulation
  • Distributed Systems and Data Platform Development
  • Molecular and genetic basis of cancer.

Technology Areas

  • AI & ML
  • AI & ML - Machine Learning Algorithms