Radiation-Induced Transformation in Human Breast Cells.
Abstract
In this study we have shown that ionizing radiation can transform immortalized/ initiated human breast epithelial cells from a donor with Li-Fraumeni syndrome (LFS) containing a germline mutation in the p53 gene to a malignant phenotype. Exponentially growing human breast cells (HME-50) were irradiated with 2 Gray of gamma radiation with additional doses of 2 Gray delivered at daily intervals to a total dose of 60 Gray. After each increment of Gray the cultures were allowed to recover for 10 7-10 days. After this recovery the cultures were tested for changes in morphology, anchorage-independent growth, growth -factor requirements, growth in the presence of serum and tumor formation in scid mice. In comparison to cultures which were unirradiated or treated with total dose of 10 and 20 Gray, HME-50O cells treated with 30-60 Gray exhibited distinct changes in cellular morphology, reduced growth factor requirements, increased cell density at confluence and anchorage- independent growth. Most significantly, they reproducibly produced tumors in scid mice at a high frequency. Further studies using clonal isolates from these radiation transformed breast cells will be required to determine the role of the remaining wild-type p53 allele in this transformation process.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA365542
Entities
People
- Peter J. Thraves
Organizations
- Georgetown University