Tumor Suppressor Genes in Early Breast Cancer and Its Progression.
Abstract
Ductal carcinoma in situ (DCIS) is a pre-invasive stage of breast cancer in which the malignant cells have not penetrated the basement membrane. From our initial allelotyping study of DCIS we determined that the background rate of loss of heterozygosity (LOH) is low (5%). The chromosomal regions showing LOH, significantly above background were 8p, 13q, 16q, 17p and 17q. in our 1996 report we described the LOH mapping of a regon on 8p (30% LOH) containing a; putative tumor suppressor gene (TSG) which we refined to a small interval of approx. 1.4 cM between the markers D8S265 to D8SD520. Last year (1997) we described continued mapping work in this region including construction of radiation hybrid maps and initial progress on assembling a YAC/BAC contig for the region. During this project year we completed construction of an integrated YAC/BAC contig which enabled more precise measurement of the physical size of the region containing the putative TSG (1240 Kb). We also constructed a fine structure radiation hybrid map for the critical region. Four cDNAs from the 1.4 cM/1240 Kb region have been identified. DNA sequencing is in progress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA366052
Entities
People
- Helen Donis-keller
Organizations
- University of Washington