A Capillary Instrument for High Speed DNA-Based Tumor Diagnosis.
Abstract
In order to test individuals for familial breast-cancer susceptibility, a fluidic 96-channel capillary DNA sequencing and analysis instrument is under development. The major subsystems of this instrument have been designed and tested. A novel manifold system will allow automated unattended loading to be performed. Ostensibly minor technical issues stand in the way of application of the prototype system to real samples on a large scale. User-friendly software is close to fully functional. The instrument will have major benefits over other commercial instruments that have been announced. Foremost is the ability to perform preparation, processing, loading, separation, detection, and analysis of DNA sequencing reactions in a coupled in-line fashion in a single instrument. Simultaneously, techniques for the practice of capillary DNA sequencing are being refined. New matrices for separating DNA have been developed and optimized. Sample purity can be assessed using a novel miniature conductivity probe. A new technique for loading samples without purification has also been developed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA366307
Entities
People
- Harold P. Swerdlow
Organizations
- University of Utah