Representational Difference Analysis of Human Breast Cancer.
Abstract
The localization of homozygous deletions has been useful in the identification of new tumor-suppressor genes and in identifying the roles of known genes. Four major approaches can identify such deletions: a candidate gene approach where losses of a particular sequence are sought, a candidate positional search where a genome scanning strategy utilizes spaced markers, a candidate locus approach where markers of a known homozygous deletion of one tumor system are extended to a second tumor type, and an unbiased approach such as the representational difference analysis (RDA). Approaches utilizing RDA, candidate genes, and candidate loci in breast cancer were explored by our group. A homozygous deletion of the DPC4 gene was identified in a breast cancer cell line, suggesting the involvement of this TGF-beta-like pathway in this cancer. Recently, we identified in a breast cancer a previously unreported homozygous deletion of the MKK4 gene, involved in stress-activated pathways. This was the first confirmation of MKK4 alterations in breast cancer and suggested that alterations of the MKK4 gene may be among the most common tumor-suppressor abnormalities in breast cancer, seen in 15% of cell lines to date.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA366587
Entities
People
- Scott E. Kern
Organizations
- Johns Hopkins University