The Effect of Radiotherapy Upon Primary Human Mammary Epithelial Cells which Harbor a Breast Cancer Susceptibility Gene
Abstract
The purpose of this work is to determine whether genomic instability following radiotherapy is increased in breast cells that harbor either a BRCA-1 or BRCA-2 mutation relative to cells which carry two normal copies of the gene. Therefore mammary epithelial cells from women at high risk for carrying either a BRCA-1 or BRCA-2 germline mutation will be compared with mammary cells from women not at risk for carrying a mutation following radiation using the following assays: growth factor independence, growth in soft agar, and tumorigenicity in vivo. The work done thus far has largely focused on characterizing the high-risk cell lines. All baseline radiation experiments have been done and the growth factor experiments have begun, which show a dependence upon EGF in unirradiated high risk (and control) cell lines. Cells have also been infected with either HPV E6 and E7 to delay senescence and conduct comparison studies between cell lines with or without a BRCA mutation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA366636
Entities
People
- Lori Pierce
Organizations
- University of Michigan