Effect of HSP27 on Human Breast Tumor Cell Growth and Motility
Abstract
This award is a Predoctoral Fellowship to support the doctoral training of Donna Egender. The goal of this research is to investigate the effects of the small stress protein, HSP27, on growth and motility characteristics of normal and tumor-derived human mammary cell lines. Our study is based on the hypothesis that HSP27 is a component of a signal transduction pathway that regulates actin microfilament dynamics. We hypothesize that cells overexpressing HSP27 will show increased motility and altered chemotactic properties, in addition to increased resistance to heat killing and certain drugs. Research completed this year includes selection of 19 clonal MDA231 breast tumor cell lines that overexpress human hsp27, assay of hsp27 expression levels, and determination of the effects of hsp27 overexpression on proliferation rates of the clonal lines and their response to heat stress and drug treatments. Experiments evaluating the effects of hsp27 overexpression on motility of breast tumor cells have been initiated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA366640
Entities
People
- Eileen Hickey
Organizations
- University of Nevada, Reno