Improvement of Cultured Keratinocyte Grafts for Burn Wounds.

Abstract

This study, "Improvement of Cultured Keratinocyte Grafts for Burn Wounds", was modified from the original proposal after notification of early termination of funding of the original three year proposal. This modified study has demonstrated that keratinocyte allografts have reduced histocompatability antigen expression when grafted to burn recipients, and that this is associated with a decrease in sensitization as measured by cytotoxic T lymphocytes (CTL's) The study also demonstrated that significant reduction of CD-4 and CD-8 lymphocyte sub-populations did not affect CTL response to allograft. Furthermore, although cytokine levels are affected by bum injury, there was no consistent cytokine cascade system that would permit modification to alter keratinocyte allograft recognition or graft survival. Early studies with keratinocyte allografts from transgenic mice deficient in histocompatability antigen did find that they had significantly reduced immunogenecity and this technology may produce a universal donor keratinocyte.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA366680

Entities

People

  • Anthony A. Meyer

Organizations

  • University of North Carolina at Chapel Hill

Tags

DTIC Thesaurus Topics

  • Antigens
  • Bandages
  • Biological Factors
  • Blood
  • Burns
  • Cells
  • Chemistry
  • Connective Tissue
  • Culture Techniques
  • Cultured Cells
  • Epithelial Cells
  • Genetic Engineering
  • Lymphatic System
  • Lymphocytes
  • Medical Personnel
  • Proteins
  • T Lymphocytes

Fields of Study

  • Medicine

Readers

  • Geochemistry
  • Immunology
  • Trauma Surgery or Emergency Medicine.