Characterization of BRCA2 Transcriptional Regulation.
Abstract
The purpose of this award is to study the transcriptional regulation of the BRCA2 breast cancer predisposition gene with the goal of identifying agents capable of modulating BRCA2 expression. The grant was originally awarded to the University of Pennsylvania, where early studies took place. Initially, reagents for transcriptional studies were generated. The promoter was cloned into luciferase reporter vectors, and expression constructs of BRCA2, BRCA1, p53, p21, p27 and a number of other cell cycle regulating genes were generated. Early' reporter experiments utilized these constructs in MCF-7 breast cancer cells. Transfection efficiency was normalized using a LacZ expressing pCHl 10 construct. Studies demonstrated that BRCA2, BRCAl, p53, p21, p27, E2F, SV4O-T had no effect on BRCA2 expression. Thus BRCA2 would not seem to be directly modulated by regulators of the cell cycle.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADA366909
Entities
People
- Fergus J. Couch
Organizations
- Mayo Clinic