Mechanisms of Integrin-Mediated Growth Control in Normal, Transformed, and Neoplastic Breast Cells
Abstract
We are interested in studying the role of integrins in the regulation of cell proliferation in normal and transformed mammary epithelial cells. During this past year, we have observed a possible difference in the abilities of integrins to stimulate cell proliferation in normal mammary epithelial cells but not in the highly metastatic carcinoma cell line MDA-MB-435. Differences in the strength of this stimulation among integrins may represent integrin-specific effects or possibly the level of cell surface expression. The mechanisms for stimulation of cell cycle progression through G1 by integrins remain unclear. To date, we have not observed significant impacts of integrin clustering on the time course of cyclin Ds expression, Rb phosphorylation, nor the association of growth factor receptors with the integrin complexes. Significant differences between normal and tumor mammary epithelial cells were observed for the expression of cyclin D2 and in the isoforms of the integrin a6, a6A, and a6B. During this next year, we hope to clarify the reason(s) for the inability of integrins to stimulate cell proliferation of MDA-MB-435 cells and the significance of the loss of cyclin D2 expression and changes in integrin a6 isoform expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA366911
Entities
People
- Elizabeth Wayner
- Richard Tamura
Organizations
- Center for Global Infectious Disease Research