Cell Type-Specific mRNA Amplification and Expression Profiling from Breast Tumor Sections
Abstract
The evolution of solid tumors involves acquisition of genetic abnormalities, which result in changes in both the set of genes expressed and the relative levels of gene expression. A variety of methods are available for ascertaining and comparing gene expression levels between normal and malignant cells. However, the increasing number of candidate genes whose expression needs to be evaluated for prognostic, diagnostic, therapeutic, or research purposes will require obtaining material from numerous tissue sections. Therefore this proposal is motivated by the need for more effective use of clinical specimens, and will address the problem of obtaining sufficient and cell type specific mRNA from clinical breast tumor specimens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA366918
Entities
People
- Donna G. Albertson
Organizations
- University of California, San Francisco