Mammary Tumor Development: Stromal-Epithelial Interactions in Oncogenesis.
Abstract
The purpose of this grant is to define interactions between stroma and epithelium in mammary oncogenesis in mice transgenic for Shope growth factor (SGF). Different lines of SGF transgenic mice express this EGF-like cytokine using inducible (metallothionein, MT) or constitutive (RSV-LTR) promoters. For the year now ending, most stated goals have been met, although some remain. We made sufficient SGF-transgenic mice of RSV-SGF and MT-SGF lines to analyze mammary differentiation and oncogenesis, including testing SGF expression and that of particular cellular genes that are important in these processes. It also induced defining which cells express the transgene and these cellular genes. We have also interbred RSV- and MT-SGF transgenic mice with p53-/- mice, to determine the nature of the interactions between homozygous deletion of p53 tumor suppressor gene and SGF expression as regards mammary gland development and tumorigenesis. These mice have been analyzed, and additional numbers of them are being produced. Our study of SGF activity in cultured cells has been progressing more slowly, and these studies are largely still in progress. Thus, our studies of the effects of SGF on the murine mammary gland are proceeding, and additional work is in progress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA367006
Entities
People
- David S. Strayer
Organizations
- Thomas Jefferson University