Genetic Toxicity Evaluation of Iodotrifluoromethane (CF3I). Volume I: Results of Salmonella Typhimurium Histidine Reversion Assay (Ames Assay)
Abstract
Under subcontract to ManTech Environmental Technology, Incorporated, Genesys Research, Incorporated, tested iodotrifluoromethane (CF(sub 3)I) using Billups-Rothenberg exposure chambers for the exposure chamber modification of the Salmonella typhimurium histidine (his) reversion mutagenesis system (the Ames test), a microbial assay that measured his right arrow his(+) reversion induced by chemicals that cause base changes or frameshift mutations in the genome of this organism. Testing was conducted using five Salmonella strains, with and without metabolic activation. CF(sub 3)I was treated in a preliminary test, which was repeated because of excessive toxicity and in a mutagenesis test. When tested over a concentration range of 1,060 to 85,908 ppm, CF(sub 3)I was found to be highly mutagenic, inducing frameshift and, particularly, base-pair mutations in Salmonella typhimurium, without and with activation. Therefore, CF(sub 3)I was positive in the Salmonella typhimurium histidine (his) reversion mutagenesis assay.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1995
- Accession Number
- ADA367277
Entities
People
- A. D. Mitchell