Role of Stroma-Derived Extracellular Matrix in Regulation of Growth and Hormonal Responsiveness of Normal and Cancerous Human Breast Epithelium.

Abstract

Extracellular matrix (ECM) proteins are critical for the differentiation of normal mammary epithelial cells, but pronounced changes in the ECM occur during breast cancer. We have determined that estrogen receptor-positive breast cancer cells (MCF-7 and T47D) cultured on laminin have reduced, or lack, estrogen responsiveness. Estrogen-induced proliferation, following anti-estrogen treatment, did not occur on laminin (T4?D) or was significantly reduced (MCF-7), but did occur on collagen I or fibronectin. Laminin inhibited basal proliferation of MCF-7, T4?D and the ER- negative MDA MB23 1 cells, but did not inhibit IGF-I or EGF induced proliferation, indicating that tumor cells on laminin do not become refractory to mitogens.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1998
Accession Number
ADA367377

Entities

People

  • Terry L. Woodward

Organizations

  • Michigan State University

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Media
  • Culture Techniques
  • Epithelial Cells
  • Indicator Dyes
  • Neoplasms
  • Peptide Growth Factors
  • Peptides
  • Proteins
  • Three Dimensional
  • Tumor Cell Line

Fields of Study

  • Biology
  • Chemistry

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics