The Role of the Cell Surface Proteases Meprin A and B in Breast Cancer Progression
Abstract
The overall objective of this project is to examine the role of the extracellular metalloproteases meprin A and B in breast cancer. The first step was to obtain full-length cDNAs encoding the human meprin and subunits and verify their expression in human cell cultures. A complete human meprin a cDNA was constructed and expressed in HEK293 cells, and it produced a functional meprin protein. This cDNA has been transfected into MCF-7 breast cancer cells under the control of a constitutive promoter. Stable MCF-7 transfectants are currently being screened for meprin expression. In addition, the human meprin a cDNA has been cloned into an inducible expression vector, and it is also being transfected into MCF-7 cells. A full-length meprin % cDNA is nearly complete and will be tested in HEK293 and MCF-7 cells. Fusion proteins containing the meprin A and B protease domains have been made and will be used as antigens for producing anti-meprin antibodies.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADA367479
Entities
People
- Gail L. Matters
Organizations
- Penn State College of Medicine