Characterization of a p53 Regulatory Domain.
Abstract
Our purpose is to characterize the proposed p53 regulatory domain and identify kinase(s) that may be involved in the regulation. Since 7/1/97, we have constructed a set of Gal4-p53N mutations and assayed their transcription activity in vivo. Our preliminary data show that stimulation by TPA reduced transcriptional activity of Gal4-p53N(delta 92-109) and Gal4-p53N(delta 117-128),- but not Gal4-p53(1-92), suggesting that residues 92-109 and 117-128 indeed play a role to reduce p53 transcription activity in response to TPA. In addition, we also show that co-transfection with MAPK but not mutant MAPK reduced p53 transcription activity, suggesting a role of MAPK in regulation of p53 transcription activity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADA367640
Entities
People
- Brian C. Abela
Organizations
- University of California, Riverside