Comparison of Two Cell-Based Assays for Screening Dioxin and Dioxin-Like Compounds in Sediments.

Abstract

The purpose of this technical note is to report the results of studies comparing two cell-based assays as screening tools for dioxins and related compounds in sediments. The Reuber H4IIE rat hepatoma cell line and the recombinant 101L human hepatoma cell line were used to assay 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalents (TEQs) in split samples of the same sediment extracts. The two methods were compared for sensitivity and correlation with analytical chemistry results. An interlaboratory comparison of results obtained with the 101L cells was performed.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1999
Accession Number
ADA367780

Tags

DTIC Thesaurus Topics

  • Analytical Chemistry
  • Cell Line
  • Cell Nucleus
  • Cells
  • Chemical Analysis
  • Chemistry
  • Environmental Protection
  • Gas Chromatography
  • High Temperature
  • Mass Spectrometry
  • Materials
  • New York
  • Polychlorinated Biphenyls
  • Sediments
  • Silica Gels
  • Spectrometry
  • Test Methods

Readers

  • Aquatic Ecology
  • Molecular Genetics
  • Oncology and Biomarker-Based Cancer Detection.