Development of a Reproductive Toxicity Test Using Xenopus
Abstract
Current reproductive toxicity tests may be missing significant toxicity by not examining the gametes of female organisms. The purpose of the present study is to develop and validate a reproductive toxicity test using the gametes and embryos of Xenopus laevis. Sexually mature female Xenopus laevis were exposed to varying concentrations of cadmium chloride (CdCl(sub 2)) in order to examine the effects of cadmium (Cd) upon oogenesis and progeny. Frogs were exposed for a period of 21 days and then either bred to untreated males or their ovaries were examined to determine effects upon oogenesis. Preliminary evaluation of the protocol for reproductive assessment appear favorable. Results of CdCl(sub 2) exposure indicated that Cd significantly disrupted the process of oogenesis. The percentage of ova were reduced at most stages, there was a significant increase in atretic oocytes, and ovaries readily accumulated Cd via subcutaneous injection of Cd into the dorsal lymph sac of females. Additionally, results indicated that Cd is transferred maternally and that the FETAX assay can be easily modified to assess the effects of maternal exposure to reproductive toxicants upon progeny. The present study provides the protocol for a reproductive toxicity assay.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA368251
Entities
People
- John A. Bantle
Organizations
- Oklahoma State University–Stillwater