Analysis of the Fission Yeast Rad3+ Gene Product
Abstract
Schizosaccharomyces pombe rad3+ is homologous to the human ATM gene, which when mutated, causes the autosomal recessive human disease ataxia-telangiectasia. A-T patients suffer from a variety of symptoms, including a predisposition to cancers such as leukemias and lymphomas. A-T heterozygotes may also have an increased risk of developing cancers, in particular breast cancer. ATM and Rad3p are both structurally and functionally similar, as cells from A-T patients and rad3- yeast mutants both have sensitivities to ionizing radiation, and defects in cell cycle checkpoints. Since fission yeast Rad3p is believed to be involved in monitoring the state of the DNA, we have investigated genetic interactions between Rad3p and DNA replication proteins by using both genetic and biochemical approaches. We have also performed a structure/function analysis of rad3+, which has identified domains important for normal rad3+ function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1999
- Accession Number
- ADA368445
Entities
People
- Carolyn R. Chapman
Organizations
- Harvard Medical School