Filtration Characteristics of MS2 Bacteriophage Using Various Molecular Weight Filters.

Abstract

MS2 Bacteriophage has a reported nominal molecular weight of 2M Dalton. It would be expected that this phage would not pass through filters of various sizes with low molecular weight cut off (MWCO) values of less than 1M Dalton. It was discovered that MS2 Bacteriophage will pass through filters with 750K Dalton, 500K Dalton, and 300K Dalton MWCO values. MS2 was retained on the 100K Dalton filter. A cross flow hollow fiber apparatus was used for the 750 and 500K Dalton analysis. Centrifuge filters of 1M and 300K and 100K Dalton were used. The rate of passage of MS2 through the cross flow filters is dependent upon the tangental flow rate and pressure. Passage through the centrifuge filters depended upon the gravitational force applied.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1999
Accession Number
ADA368535

Entities

People

  • Charles H. Wick
  • Patrick E. Mccubbin

Organizations

  • Edgewood Chemical Biological Center

Tags

Communities of Interest

  • Counter WMD

DTIC Thesaurus Topics

  • Bacteriophages
  • Biological Sciences
  • Buffers (Chemistry)
  • Centrifuges
  • Cross Flow
  • Detection
  • Detectors
  • Dilution
  • Filters
  • Filtration
  • Flow
  • Materials
  • Molecular Weight
  • Particle Size
  • Technical Information Centers
  • Ultrafiltration
  • Viruses

Readers

  • Analytical Chemistry
  • Molecular Genetics
  • Space Exploration and Orbital Mechanics.