Removing Complex Growth Media from MS2 Bacteriophage Cultures.

Abstract

Purification and concentration of viruses from the background material are required whatever the subsequent analysis methods used. For the analysis of viruses, it is essential, and detection methods depend on this solution. This report demonstrates a methodology for removing growth media from a virus preparation. A sample of MS2 was purified using a new ultrafiltration (UF) technique with hollow fibers. A typical MS2 virus sample with a nominal stated concentration of 1.4x10(exp 12) plaque forming units per ml (pfu/ml) in the original growth media was used to demonstrate this method. After UF, the growth media was removed and the viruses counted using the Integrated Virus Detection System (IVDS) instrument. This report further describes the use of this ultrafiltration procedure to remove other impurities, such as cesium chloride and albumin, from solutions containing a purified solution of MS2 bacteriophage. These solutions were also analyzed using the IVDS instrument.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1999
Accession Number
ADA368537

Entities

People

  • Charles H. Wick
  • Patrick E. Mccubbin

Organizations

  • Edgewood Chemical Biological Center

Tags

Communities of Interest

  • Counter WMD

DTIC Thesaurus Topics

  • Abstracts
  • Analyzers
  • Bacteriophages
  • Biological Sciences
  • Buffers (Chemistry)
  • Chlorides
  • Cross Flow
  • Detection
  • Detectors
  • Filtration
  • Flow
  • Impurities
  • Materials
  • Molecular Weight
  • Particle Size
  • Particles
  • Ultrafiltration

Fields of Study

  • Biology
  • Medicine

Readers

  • Materials Science and Engineering.
  • Mathematics or Statistics
  • Virology (or Medical Virology).