Alternative DNA Damage Checkpoint Pathways in Eukaryotes.
Abstract
The goals of this grant are to perform a mutagenesis screen to identify the genes in the alternative DNA damage checkpoint pathway in yeast, to characterize and clone these genes, and to isolate and analyze the human counterparts of these genes. During the first year of this grant, we have constructed a cdc9-8, rad9 (delta) double mutant strain SCP2, which is both temperature-sensitive at 30 deg C and UV-sensitive, for the mutagenesis screen. Approximately 220,000 colonies of SCP2 have been mutagenized by EMS and screened for temperature-sensitivity at 30 deg C. This primary screen yields three temperature-sensitive mutants, chb13, chb16, and chb57. A secondary UV sensitivity screen was also performed on these three chb mutants. In summary, chb13 is a strong mutant that can no longer be rescued by CHES1 in both temperature and UV sensitivity. The other two mutants have only one strong phenotype. Specifically, chb16 is highly temperature sensitive but only moderately UV sensitive, whereas chb57 is very sensitive to UV but not as sensitive to high temperature. Our next goal is cloning the genes mutated in these three strains.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1999
- Accession Number
- ADA369305
Entities
People
- Yi-chen J. Li
Organizations
- Baylor College of Medicine