Molecular Mechanisms of Metastasis Suppression in Human Breast Cancer.
Abstract
The major cause of cancer deaths can be attributed to metastasis. Our goal was to identify metastasis-controlling genes for human breast cancer. This research is based upon our finding that microcell-mediated transfer of chromosome 11 into MDA-MB-435 results in nearly complete suppression of metastasis without suppressing tumorigenicity. The key findings during this reporting period were: (1) identification of three novel genes which are more highly expressed in metastasis-suppressed human breast carcinoma cell lines; (2) demonstration that one of the novel genes, designated BrMS1 (Breast Metastasis Suppressor-1), suppresses breast cancer metastasis in a dose-dependent manner; (3) BrMS1 maps to 11q13, a site commonly altered in late-stage breast carcinoma; (4) Preliminary results include: (1) Full-length for one of the two other novel cDNAs has been cloned. Transfections have been initiated and colonies are beginning to appear. Despite several attempts, we have been unsuccessful in obtaining full-length for the third novel gene. In short, we have essentially completed the major objective of this proposal and are completing initial characterization of the candidate metastasis suppressor gene.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA371153
Entities
People
- Danny R. Welch
Organizations
- Penn State College of Medicine