Carbohydrate Mimicking Peptides as Inhibitors of Angiogenesis and Metastasis
Abstract
Sialyl-Lewis a (SA-Lea) and its structural isomer Sialyl-Lewis X (SA-LeX) carbohydrates are expressed on leukocytes and carcinoma cells and are E-selectin ligands. A SA-Lea mimicking peptide with the sequence DLWDWFVGKPAG was previously identified by us by screening a combinatorial peptide library using a SA-Lea specific monoclonal antibody (MAb) NS19-9. In further studies, peptide DLWDWFVGKPAG was identified using peptide array which showed higher MAb binding affinity as compared to the lead peptide. We demonstrated that a peptide mimics of SA-Lea mediate a 30% and 50% inhibition of neutrophil recruitment in acute inflammatory conditions and lung colonization of tumor cells in an in vivo murine tumor model, respectively. Similar effect was achieved in E-selectin deficient mice, where metastases were completely abrogated using our established tumor model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1999
- Accession Number
- ADA371263
Entities
People
- Magdalena Blaszczyk-thurin
Organizations
- University of Pennsylvania