Poly (Methyl Methacrylate) (PMMA) and Polyactic Acid Nanoparticles as Adjuvents for Peroral Vaccines

Abstract

The possibility of an oral vaccination with deglycosilated chain-A ricin (DGCA)-nanoparticles was investigated. Oral vaccination with DGCA in form of a solution or adsorbed to poly(methyl methacrylate) nanoparticles (PMMA nanoparticles) suspended in an aqueous medium leads to an about 5/% protection of mice after an aerosol challenge with ricin. All other nanoparticle products, including suspension in a hydrophobic vehicle (paraffin) or the employment of polylactic acid did not achieve a significant protection. Protection did not correlate at all with ELISA antibodies against ricin after oral as well as subcutaneous vaccination. Peroral absorption studies with (125)I-deglycosilated chain-A ricin using auto radiography as well as liquid scintillation counting indicated a slightly higher but short-lived uptake into the residual body after adsorption of the antigen to PMMA nanoparticles suspended in saline.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1999
Accession Number
ADA372152

Entities

People

  • Jorg Kreuter

Tags

DTIC Thesaurus Topics

  • Adsorption
  • Albumins
  • Alkanes
  • Alkenes
  • Antibodies
  • Aqueous Solutions
  • Biodegradable Plastics
  • Biomedical And Dental Materials
  • Chemistry
  • Emulsions
  • High Pressure
  • Materials
  • Methacrylates
  • Nanoparticles
  • Particles
  • Physical Properties
  • Vaccination

Readers

  • Critical Infrastructure Protection in CBRN and WMD Threats.
  • Nanofabrication and Microfabrication.
  • Nuclear and Radiation Engineering.

Technology Areas

  • Biotechnology