Evaluation of Drug and Vaccine Candidates in the Human Malaria/Aotus Monkey Model.
Abstract
An AMRU-1 strain of Plasmodium vivax reverted to chloroquine resistance when selectively passaged in Aotus monkeys. A C2A clone of a Mefloquine resistant Plasmodium falciparum strain was adapted to splenectomized Aotus. Chloroquine resistance reversal was achieved in 2/3 Aotus infected with the AMRU-1 strain of P. vivax using Chloroquine at 1Omg/kg in combination with 20 mg/kg of Prochlorperazine. Oligodeoxynucleotides when given intramuscularly to Aotus improve immunogenicity of a P. falciparum PADRE 45 peptide immunogen. Intradermal reimunization with an EBA-1 75, AMA-i, MSP-1 DNA vaccine as a combination with aGM-CSF, partially protected Aotus in a rechallenge with a P. falciparum FVO strain. Neither, Aotus immunized intramuscularlly with EBA-1 75, AMA-i, MSP-i DNA vaccine as a combination with aGM-CSF, nor Aotus immunized with P. vivax Sal- 1 blood stage DNA vaccines were protected against an homologous challenge. A significant degree of strain-trascending immunity was observed in Aotus that were repeatedily challenged with an FVO strain of P. falciparum and then reinfected with an heterologous CAMP strain of P. falciparum. Aotus that were immunized with an EBA-i 75, AMA-i and MSP-i DNA vaccine intradermally as a combination were not protected when rechallenged with an FVO strain of P. falciparum.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1999
- Accession Number
- ADA373724
Entities
People
- Nicanor Obaldia Iii.