A Solid Support Synthesis and Novel Conjugation Methods of Breast Tumor Associated Antigen: Toward the Development of Cancer Vaccines
Abstract
The immunological study of globo-H, a breast tumor associated carbohydrate antigen, has been producing promising results. As the demand for this antigen grows, the efficient production has become an urgent problem. We have been trying to solve this problem via two approaches: the development of a more efficient conjugation method and the solid phase synthesis of globo-H. A solution to the conjugation problem, new method utilizing MMCCH linker, was developed to significantly improve the coupling of synthetic glycosides to the carrier protein. As for solid phase synthesis of globo-H, much insight has been gained on glycosidations on solid support, while the synthesis is still in progress. As a result, the trisaccharide intermediate of globo-H was successfully synthesized on the solid support, thus resulted in overall improvement in the synthetic efficiency.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA373924
Entities
People
- Hyunjin M. Kim
- Samuel Danishefshky
Organizations
- Memorial Sloan Kettering Cancer Center